Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. The same beta subunits also occur in Inhibins as part of a heterodimer with an alpha subunit. The beta subunits are translated as a precursor form that includes an N-terminal pro region which is proteolytically cleaved to yield the mature C-terminal beta subunit.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin A precursor is ~15ng/well. Western Blot: 1- 2ug/ml. The detection limit for recombinant human Activin A precursor is ~5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse myeloma with B cells obtained from a mouse immunized with a DNA construct encoding the human Activin A pro region fused to mature human Activin beta B chain.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
DNA construct encoding human Activin A pro region fused to mature human Activin beta B chain
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Activin A Precursor, specifically in the human Activin A pro region that is removed when the protein is processed to its mature form.
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