USBio Logo

A0868-35 Mouse Anti-Adeno-Associated Virus 2 (AAV2)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG3
Clone Number
5F148 (A20)
Grade
Affinity Purified
Applications
DB E IF IHC IP N
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
4°C Do not freeze

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Neutralization Assay, Affinity chromatography, Dot Blot and ELISA. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:20 Dot Blot: 1:500 Immunoprecipitation: 1:5 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Adeno-associated virus capsid proteins and virus particles.
Form
Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes AAV-2. Species Crossreactivity: human and monkey. Reacts with AAV-3 in ELISA. Specifically reacts wtih intact AAV particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids not present in denatured capsid proteins and native but unassembled capsid proteins. Major reaction attributed to aa369-378 of AAV-2 capsids.
References
1. Wistuba A.,Weger S., et al., (1995). J. Virol. 69, 5311-5319. 2. Wistuba A., Kern A., et al., (1997) J. Virol. 71, 1341-1352. 3. Grimm D, Kern A, et al., (1999) Gene Ther 6(7) 1322-1330. 4. Wobus C.A., Hügle-Dörr B., et al., (2000) J. Virology 74, 9281-9293. 5. Naumer M, Sonntag F, et al., J Virology 86, 13038-13048 (2012). PMCID: PMC3497687; doi: 10.1128/JVI.01675-12||Reference by Application:|Publication|Species|Application|Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014).|AAV2|AAV3|dot blot|Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038–13048 (2012).|AAV2|ELISA|dot blot|Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).|AAV2|ELISA|ICC-IF|Moskalenko, M. et al. Epitope mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761–1766 (2000).|AAV2|neutralization|Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000)|AAV2|AAV3|neutralization|ELISA|Grimm, D. et al. Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Ther. 6, 1322–30 (1999).|AAV2|ELISA|dot blot|IP|ICC-IF|Bartlett, J. S., Kleinschmidt, J., Boucher, R. C. & Samulski, R. J. Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(ab’gamma)2 antibody. Nat. Biotechnol. 17, 181–6 (1999).|AAV2||Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341–1352 (1997).|AAV2|ICC-IF|IP|Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311–5319 (1995).|AAV2|IP
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved