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A2298-51T Rabbit Anti-AP2S1 (Adaptor-related Protein Complex 2 sigma 1 Subunit, AP2 Complex Subunit sigma 1, AP-2 Complex Subunit sigma 1, AP 17, AP17, AP17 delta, Clathrin Assembly Protein 2 Small Chain, Clathrin-associated/assembly/adaptor Protein Small 2, CLAPS2, Clathrin Coat Assembly Protein AP17, Clathrin Coat-associated Protein AP17, HA2 17kD Subunit, Plasma Membrane Adaptor AP-2 17kD Protein)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P53680
Isotype
IgG
Clone Number
9H292
Grade
Supernatant
Applications
FC IP WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Adapter-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles.It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1).AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (1, 2).

Applications
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Not recommended for use in Immunocytochemistry or Immunohistochemistry (paraffin sections). Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:200 Western Blot: 1:1000-1:5000. Expected to detect a band at ~17kD. Immunoprecipitation: 1:10 Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Fetal brain lysate, HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Immunogen
A synthetic peptide corresponding to residues in human AP2S1.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Purity
Supernatant
Specificity
Recognizes human AP2S1. Species Crossreactivity: mouse and rat.
References
1. Kirchhausen T, et al. J Biol Chem. 266(17):11153-7, 1991 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)
USBio References
No references available
Conjugates
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