Apaf-1

Brand

MaxLight™

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Conjugate

MaxLight™550

Isotype

IgG

Grade

Affinity Purified

Applications

Crossreactivity

Ce Hu Ma Mo

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications

Western Blotting: 1:1000-1:2000 Optimal working dilutions to be determined by researcher.

Note: Applications are based on unconjugated antibody.

Immunogen

Synthetic peptide corresponding to amino acids 12-28 of human Apaf1.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purity

Purified IgG prepared by affinity chromatography.

Specificity

Apaf1 (Apoptosis protease activating factor 1) is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Recognizes a 130kD band in Western blots. Species Crossreactivity: Human, mouse

References

1. Zou, H. et al. (1997). Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90: 405-413.2. Cecconi, F. et al. (1998). Apaf-1 (CED-4 homolog) regulate