APOA-1 is a major protein of plasma HDL. It participates in the reverse transport of cholesterol from tissues to the liver for excretion. APOA1 is a cofactor for LCAT, which is responsible for the formation of most plasma cholesteryl esters.
Applications
Suitable for use in FLISA and RIA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Affinity Constant
5.0 x 10e6L/M. Determined by RIA followed by Scatchard Plot analysis.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human high density lipoprotein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Apolipoprotein A-I (Apo A-I). This antibody does not crossreact with Apo A-II or Apo B in competitive RIA.
TDS
NCBI
UniProt
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