ART2.2 is expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (1).

Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.

Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.

Spleen cells from immunized wistar rats were fused with cells of the Sp2/0 mouse myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

pME.CD8LF-ART2.2.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes the gene product of the mouse ADP-ribosyltransferase (ART) 2.2 gene, an ortholog of rat RT6.