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B0003-64R Goat Anti-BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q9Y275 |
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_006564
Shipping Temp
Blue Ice
Storage Temp
-20°C

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF-and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA (5). BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N-terminal kinase (JNK) and to induce apoptosis.

Applications
Suitable for use in Sandwich ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Sandwich ELISA: 0.5-2ug/ml using 100ul/well. Allows the detection of 0.2-0.4ng/well of recombinant human BAFF. Western Blot: 0.1- 0.2ug/ml. The detection limit is 1.5-3ng/lane of recombinant human BAFF under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to human BAFF, expressed in E. coli
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human BAFF.
Conjugates
Pricing
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