Beta Defensin-3 is a novel 5-9kD nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis).
Applications
Suitable for use Western Blot. Other applications not tested.
Western Blot
Tricine-SDS-PAGE gels: 1:500-1:1000. Migrates as a 5-6kD band. Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kD band. The larger size is because of the reduction of the three cysteine bridges present in the peptide protein. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide consisting of aa 23-33 (GIINTLQKYYC) of the human beta-defensin-3 protein
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the 5kD human Defensin-3, beta protein. Species Crossreactivity: human.
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