Domain Information: CBL proto-oncogene N-terminal domain 1; CBL proto-oncogene N-terminus, EF hand-like domain; CBL proto-oncogene N-terminus, SH2-like domain; Ring finger; Ubiquitin associated domain

Gene Ontology Terms: signal transducer activity; transcription factor activity; cell growth and/or maintenance; cell surface receptor linked signal transduction; nucleus; ligase activity

Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 120kD band seen in 3T3 lysate. Recommended for use at 1-3ug/ml.

Staining (2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Corresponding peptide is available for Peptide Blocking studies. See C0013-26D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

C-REFVSISSPAHVAT, C Terminus

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes Human, Mouse, Rat CBL