Western Blotting: 1:500-1:1000 Optimal working dilutions to be determined by researcher.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to amino acids 205-222 of mouse CAD.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified IgG prepared by affinity chromatography.
Specificity
CAD (Caspase Activated Deoxyribonuclease) is the mouse homologue of human DFF-40.CAD is released and activated by caspase cleavage of ICAD and eventually causes degradation of nuclear DNA. Recognizes the 40kD CAD in Western blots.
References
1. Enari, M. et al. (1998). A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391: 43-50.2. Sakahira, H. et al. (1998). Cleavage of CAD inhibitor in CAD activation and DNA degradati
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