Calmyrin is identified as a calcium-binding protein with a molecular weight of ~22kD. Calmyrin displays various properties, including myristoylation, membrane-association, and colocalization with presenilin-2 (PS-2). Functionally, like presenilin, calmyrin causes cell death when overexpressed in HeLa cells. Interestingly, calmyrin coexpresses with PS-2, and induces additive effects in apoptosis. These results indicate diverse roles of calmyrin in cell signaling, cell death and Alzheimer disease through the modulation of presenilin function.

Suitable for use in FLISA and Western Blot. Other applications have not been tested.

FLISA: 0.1–1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Recombinant Calmyrin protein (full length)

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by Protein A chromatography

Reacts with both overexpressed and endogenous Calmyrin protein. On Western blots, this antibody identifies a band at ~22kD. Reactivity has been confirmed with human HeLa cell lysates.