Carabin is a 50kD protein belonging to the TBC1 domain family of proteins. Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes.

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.

Human spleen cell lysates

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to a 17aa from near the amino terminus of human Carabin.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by affinity chromatography.

Recognizes human Carabin at ~55kD. Does not crossreact with either TBC1D10A or TBC1D10B. Species Crossreactivity: mouse