Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3– + H+ , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: Neat. 10ul labels 1x10e6 cells. Tested using U-87 MG human glioblastoma/astrocytoma cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Pro59-Asp414 of human Carbonic Anhydrase IX (CA9) expressed in NS0 cells
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human Carbonic Anhydrase IX (CA9). Does not crossreact with recombinant mouse CA9 or with recombinant human CA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13 or 14.
TDS
NCBI
UniProt
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