Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested.

FLISA: 0.1-1.0ug/ml Western Blot after IP: 1-2ug/ml. Crossreactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10ug. Specifically immunoprecipitate the ~ 92kD b-Catenin protein from native cell lysates. Optimal dilutions to be determined by the researcher.

HeLa and A431 lysates.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by immunoaffinity chromatography.

Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..