Suitable for use in Flow Cytometry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

1. Five x 10e5 ficoll prepared human peripheral blood lymphocytes were washed and pre incubated 45 minutes with 80ul of C2267-33A at 1ug/ml. Cells were washed twice and incubated with  IgG (FITC) Goat anti-Mouse, after which they were washed three times, fixed and analyzed by FACS. A net 14% sub population of the cells stained positive with a mean shift of 1.8 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human polymorphonuclear leukocytes.

Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.

Purified by Protein A affinity chromatography from tissue culture supernatant.

Recognizes both allelic forms of CD16. Antibody blocks binding of complexed IgG to CD16.