Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:1000-1:5000. The epitope recognized by this antibody is sensitive to formaldehyde fixation and tissue processing. Acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections. Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control
Human Tonsil
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human neutrophils
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified from tissue culture supernatant
Specificity
Recognizes human CD18. Species Crossreactivity: canine and guinea pig. Demonstrates strong reactivity with leucocytes and is not reactive with platelets.
References
1. Bindon C.I.et al (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. Eur. J. Immunol. 18: 1507-1514, 2. Galfre et al. (1979) Rat X rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature.277: 131-133, 3. Chabanne, L. et al. (1994) Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes. Tissue Antigens 43: 202-205.
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