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C2269-83K Mouse Anti-CD19 (Cy5)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Cy5
Isotype
IgG1,k
Clone Number
5K218 (SJ25-C1)
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR).1-5

Applications
• Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation
Recommended Dilution
Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NALM-1 and NALM-16 leukemia cell line.
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Cy5.
Purity
Purified
Specificity
Recognizes human CD19.
References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD19 Section, Academic Press, New York, p. 179.|3. Tedder, T.F., L.J. Zhou, and P. Engel. 1994. Immunol. Today 14:437.|4. Fearon, D.T., and R.H. Carter. 1995. Annu. Rev. Immunol. 13:127.|5. Doody, G.M., P.W. Dempsey, and D.T. Fearson. 1996. Curr. Opin. Immunol. 8:378.
USBio References
No references available
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