CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. Membrane-spanning 4-domains, subfamily A, member 1, also known as MS4A1, is a human gene. This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. The protein has no known natural ligand and its function is unclear. It is suspected that it acts as a calcium channel in the cell membrane.
CD20 is expressed on all stages of B cell development except the first and last; it is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, and B-cell chronic lymphocytic leukemia. It is also found on skin/melanoma cancer stem cells. CD20 is the target of the monoclonal antibodies rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher.
Recommended Negative Control
I1904-84H3: IgG2b Negative Control (PE)
Storage and Stability
Lyophilized powder may be stored at 4°C. Reconstitute by adding ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CD20 cell surface antigen, a 33-37kD phosphoprotein. CD20 is expressed by B lymphocytes and by a small subset of normal circulating T lymphocytes. Species Crossreactivity: Rhesus Monkey.
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