CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on granulocytes and monocytes.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: Acetone-fixed, frozen tissue sections only Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS containing 0.09% sodium azide. Labeled with Biotin
Specificity
Recognizes porcine CD21, Mr 160kD. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer’s patches (ILPP); 93-97% in spleen; and 0% in thymus. The antibody does not stain B cells in cord blood or spleen from porcine fetuses (105 days gestation).
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