Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested.

Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Burkitt's lymphoma cell line Raji.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purified by Protein A affinity chromatography from tissue culture supernatant.

Recognizes the human CD22 cell surface antigen, a 130/140kD glycoprotein expressed by cells of B lymphocyte lineage.