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C2275-82A Rat Anti-CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
FITC
Isotype
IgG2b,k
Clone Number
3C7
Grade
Purified
Applications
FC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.5 It is expressed at a higher level on CD4+CD8+ thymocytes.5, 6 It is also expressed on cultured epidermal Langerhans cells.7 The biochemical features of murine CD25 have been characterized in detail.8-10. This antibody, used in combination with C2275-89A in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. 2, 13

Applications
• Identification and enumeration of CD25+ cells by flow cytometry 1-4 • Immunoprecipitation 1, 2, 8, 10 • Inhibition of IL-2 or mitogen-induced T cell proliferation 13 • In vitro blocking of IL-2 binding 2, 12
Recommended Dilution
Flow Cytometry: ≤ 3ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Purity
Purified
Specificity
Recognizes the low affinity alpha chain IL-2 receptor, Mr 55kD (IL-2Ra).
References
1. Malek, T. R., R. J. Robb, and E. M. Shevach. 1983. Proc. Natl. Acad. Sci. U. S. A. 80:5694.|2. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970.|3. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405.|4. Andrew, M. E., A. M. Churilla, T. R. Malek, V. L. Braciale, and T. J. Braciale. 1985. J. Immunol. 134:920.|5. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265.|6. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456.|7. Steiner, G., E. Tschachler, M. Tani, T. R. Malek, E. M. Shevach, W. Holter, W. Knapp, K. Wolff, and G. Stingl. 1986. J. Immunol. 137:155.|8. Malek, T. R. and P. E. Korty. 1986. J. Immunol. 136:4092.|9. Malek, T. R., J. D. Ashwell, R. N. Germain, E. M. Shevach, and J. Miller. 1986. Immunol. Rev. 92:81.|10. Saragovi, H. and T. R. Malek. 1988. J. Immunol. 141:476.|11. Lorenzo, F., C. Jaulin, N. Vita, P. Froussard, P. Ferrara, D. L. Jankovic, and J. Theze. 1991. J. Immunol. 147:2970.|12. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929.|13. Malek, T. R., G. Ortega, J. P. Jakway, C. Chan, and E. M. Shevach. 1984. J. Immunol. 133:1976.|14. Leonard, W.J., et. al. 1984. Nature 311:626|15. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257
USBio References
No references available
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