Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.

NS1 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Con A stimulated rat T cells.

Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).

Purified by Protein G affinity chromatography.

Recognizes the rat CD25 cell surface antigen, a 55kD glycoprotein which acts as a low affinity receptor for IL-2.CD25 is expressed by activated T lymphocytes.