CD41, CD61

Clone Type

Monoclonal

Host

Mouse

Source

Sheep

Isotype

IgG1

Clone Number

10B1564 (CO.35E4)

Grade

Affinity Purified

Applications

E FC IP

Crossreactivity

Bo Ca Eq Fe Gt Rb Sh

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD41/CD61 is also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis.

Applications

Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions

Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

Recommended Negative Control

I1904-78R: IgG1 Murine Negative Control

Hybridoma

Sp2-0/AG14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Sheep leukocytes

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes sheep CD41/CD61. Species Crossreactivity: goat, feline, rabbit, canine, equine and bovine

References

1. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet. Immunol. Immunopathol. 64: 1-13. 2. Pintado, C. O. et al. (1995) Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leucocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/CD61). Vet Immunol. Immunopathol. 44 (3-4): 359-368. 3. Lalko, C. et al. (2003) Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study. Vet. Immunol. Immunopathol. 91: 119-134. 4. Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer. Biomaterials. 31: 2736-45. 5. Brainard, B.M. et al. (2012) Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates. Vet Immunol Immunopathol. 149: 119-25. 6. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO). Vet Immunol Immunopathol. 164 (1-2): 87-92.

USBio References

No references available

Product	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

C2394-35A1 Mouse Anti-CD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex)

Clone Type

Host

Source

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

References

USBio References

Conjugates

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods