Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:100-1:1000 Immunohistochemistry (Paraffin): 1:100-1:1000. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilution determined by the researcher.
Recommended Secondary Reagents
Goat anti mouse IgG:HRP (rat adsorbed) Goat anti mouse IgG:RPE (rat adsorbed)
Recommended Negative Controls
I1904-78Y IgG1, Murine Negative Control (For rat tissue)
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHA stimulated rat lymphocytes
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat CD45C., the high molecular weight form of the leucocyte common antigen found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells but splits CD4+ve T cells into two populations differing in their helper activities.
References
1. Spickett, G.P. et al. (1983). J. Exp. Med. 158: 795-810. 2. Standring, R. et al. (1978). Eur. J. Immunol. 8: 832-839. 3. Barclay, A.N. et al. (1987). EMBO J. 6: 1259-1264. 4. Ralph, S.J. et al. (1987). EMBO J. 6: 1251-1257. 5. Arther, R.P. & Mason, D.W. (1986). J. Exp. Med. 163: 774-786. 6. Barclay, A.N. (1981). Immunology 42: 593-600. 7. McCall et al. (1992). Immunology 76: 31. 8. Syster et al. (1994). Int. Imm. 6: 1875.
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