CD47 is a cell surface glycoprotein that is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.

Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications have not been tested.

Immunohistochemistry: Frozen sections Western Blot: Recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction Optimal dilutions to be determined by researcher.

I1904-84Q3: IgG2b Negative Control

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Human erythrocytes

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes the human CD47. Species Crossreactivity: porcine, canine, sheep, bovine