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C2402-04-ML490 Mouse Anti-CD47 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Swiss Prot
P97829
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
3H1457 (OX-101)
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilution determined by the researcher.
Hybridoma
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Recommended Secondary Reagents
IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed)
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat thymocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.
References
1. Vernon-Wilson, E. F. et al. (2000). CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRP alph a 1. Eur. J. Immunol. 30: 2130-2137. 2. de Vries, H.E. et al. (2002). Signal regulatory protein alpha - CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J. Immunol. 168: 5832 - 5839.
USBio References
No references available
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