MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
The integrin alpha 2 subunit is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from immunized mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human integrin alpha 2 subunit expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. Species Crossreactivity: cynomolgus monkey, rhesus monkey, baboon
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