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C2404-65A Rat Anti-CD49d (Integrin alpha4, VLA4, LHR, Lymphocyte Homing Receptor)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
Q00651, P13612
Isotype
IgG2b,k
Clone Number
5K278 (PS/2)
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors (1-3). As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1 (1,2). In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1 (2,3). CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive (1,2).

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
P815 cells
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity
Purified
Specificity
Recognizes mouse CD49d. Species Crossreactivity: human.
References
1. Miyake, K., I. L. Weissman, J. S. Greenberger, and P. W. Kincade. 1991. J. Exp. Med. 173:599.|2. Christensen, J. P., E. C. Andersson, A. Scheynius, O. Marker, and A. R. Thomsen. 1995. J. Immunol. 154:5293.|3. Henseleit, U., K. Steinbrink, C. Sunderkotter, M. Goebeler, J. Roth, and C. Sorg. 1994. Exp. Dermatol. 3:249|4. .Lauri, D., C. De Giovanni, T. Biondelli, E. Lalli, L. Landuzzi, A. Facchini, G. Nicoletti, P. Nanni, E. Dejana, and P. L. Lollini. 1993. Br. J. Cancer 68:862.
USBio References
No references available
Conjugates
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