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C2405-06D Mouse Anti-CD50 (Intercellular Adhesion Molecule, ICAM-3, Lymphocyte Function-Associated Antigen-1, LFA-1) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG2b
Clone Number
7H110
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Human CD50 is an adhesion molecule that interacts with LFA-1 (CD18/CD11a) and provides a costimulatory signal for T cells which is thought to be essential for initiation of immune responses.

Functional Applications: Antibody blocks binding of CD11a (LFA-1) to CD50 (ICAM-3)
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Immunogen
Stimulated human leukocytes
Form
Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the D1 domain of the ICAM-3 molecule of 130kD.
References
1) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579. |2) C.L. Holness, et al, (1995) J Biol Chem 270: 877-884. |3) Y. van Kooyk, et al, (1996) J Exp Med 183: 1247-1252. |4) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.
USBio References
No references available
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