CD51, a 140kD alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61. Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing.

Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.

Spleen cells from immunized SD rats were fused with the cells of the P3X.63Ag8.653 myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Cultured LAK cells from Balb/c mice.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes mouse CD51. Reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.