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C2406-13 Mouse Anti-CD50 (Intercellular Adhesion Molecule, ICAM-3, Lymphocyte Function-Associated Antigen-1, LFA-1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
101-1D2
Grade
Supernatant
Applications
IC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD50 is a single chain polypeptide with a MW of 120kD. The protein is heavily glycosylated and resistant to treatment with phosphatidylinositol phospholipase C, and therefore probably not PI-anchored to the membrane. The antibody was provisionally clustered as CDw50 in Vienna at the IV and as CD50 in Boston at the V international workshop on human leukocyte differentiation antigens.

Applications
Suitable for use in Immunohistochemistry (frozen sections; paraffin-embedded tissue after microwave treatment, and cell preparations) and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:10; Incubation Time 30 min. at RT. Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Isolated from human leukocytes
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Culture supernatant
Specificity
Reactive with human. Reacts with the cellular adhesion molecule CD50 (ICAM-3).
References
Vilella R et al. Involvment of the CDw50 molecule in allorecognition. Tissue Antigens 36, 203-210 (1990)|Knapp W et al. Leucocyte typing IV, Oxford Univ Press, pp 541, 667-670, 1087. (1989)|Schlossman et al. Leukocyte typing V, Oxford Univ Press, pp 1546-1547, 2011, (1993)|Pino-Otin MR et al. Existence of a soluble form of CD50 (ICAM-3) produced upon human lymphocyte activation. J Immunol 154, 3015-3024 (1995)
USBio References
No references available
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