Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

ELISA: Capture Immunohistochemistry: Fixed cells and tissue Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher.

1. Five x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of C2409-11M at 1ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3.Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human monocytes

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.

Purified by Protein A affinity chromatography.

Recognizes the D1 domain of human CD54 at~90kD. Inhibits CD54 binding to LFA-1.