USBio Logo

C2410-01E-Biotin Mouse Anti-CD55 (CD55 Antigen, CD55 Molecule, Complement Decay Accelerating Factor, CR, Cromer Blood Group Antigen, Cromer Blood Group System, Decay Accelerating Factor for Complement, DAF, TC) (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P08174
Conjugate
Biotin
Isotype
IgG1
Clone Number
3H1563 (67)
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

CD55 cell surface antigen is a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-hemopoietic tissues, particularly epithelium and is specifically found at the fetal-maternal interface in placenta (Holmes et al. 1990 and Yang et al. 2009). Soluble forms of DAF are found, for example, in plasma, saliva and urine (Medof et al. 1987). The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Not recommended for staining erythrocytes. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Positive Control
Human tonsil
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
K562 cells
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Specificity
Recognizes human CD55.
References
1. Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W. et al. Oxford University Press pp 694-697, 2. Lublin D.M. and Atkinson J.P. (1989) Decay-accelerating factor: biochemistry, molecular biology and function. Ann. Rev. Immunol. 7: 35-58, 3. Daniels G.L. (1989) Cromer-related antigens - blood group determinants on decay-accelerating factor. Vox Sang. 56: 205-211, 4. Holmes C.H. et al. (1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy. J. Immunol. 144: 3099-3105.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • CD55
    C2410-01E-Biotin Mab
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved