CD55

Clone Type

Monoclonal

Host

Mouse

Source

Human

Conjugate

PE/CY5

Isotype

IgG1

Clone Number

143-30

Grade

Purified

Applications

FC IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD55, also known as decay accelerating factor (DAF), is a 64-73kD GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum, including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.1-5

Applications

• Flow cytometry • Immunohistochemistry (frozen sections)

Recommended Dilution

Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.

Form

Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.

Purity

Purified

Specificity

Recognizes human CD55.

References

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD55 Section, Academic Press, New York, p. 281.|3. Lublin, D.M., and J.P. Atkinson. 1989. Annu. Rev. Immunol. 7:35.|4. Holmes, C.H., K.L. Simpson, S.D. Wainwright, C.G. Tate, J.M. Houlihan, I.H. Sawyer, I.P. Rogers, F.A. Spring, D.J. Anstee, and M.J. Tanner. 1990. J. Immunol. 144:3099.|5. Liszewski, M.K., T.C. Farries, D.M. Lublin, I.A. Rooney, and J.P. Atkinson. 1996. Adv. Immunol. 61:201.

USBio References

No references available

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C2410-50E Mouse Anti-CD55 (DAF, Decay Accelerating Factor) (PE/CY5)

Clone Type

Host

Source

Conjugate

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

References

USBio References

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