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C2410-55B Mouse Anti-CD56 (NCAM-1, Neural Cell Adhesion Molecule) (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1
Clone Number
7H185
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses.

Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (frozen): acetone fixation Flow Cytometry: 10ug/ml will label 10e5 cells Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1. Five x 10e5 ficoll prepared human peripheral white blood cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80ul of C2410-55B at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with Streptavidin (PE) (Catalog #S7973-88P). 3. After which they were washed three times, fixed and analyzed using by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 (Biotin) negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD56 antibody (Catalog #C2410-55A).
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human retinoblastoma tumor tissue, membrane fraction
Form
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD56 molecule found on NK cells.
References
1. S.P. Bourne, et al, (1991) J Neuro-Oncol 10: 111-119. 2. T.L. Whiteside and R.B. Herberman (1994) Immunology 1: 125-133. 3. H. Spits, et al, (1995) Blood 85: 2654-2670. 4. R. Gerardy-Schahn, M Eckhardt, (1994) Int J Cancer: Supp 8: 38-42.
USBio References
No references available
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