Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.

Flow Cytometry: 10ug/ml (80ul) labels 5x10e5 cells Optimal dilutions to be determined by the researcher.

1. Five x 10e5 ficoll prepared from human peripheral blood mononuclear cells per tube were washed and preincubated for ~5 minutes with 20ul of 300ug/ml human IgG (to block nonspecific binding). 2. They were for incubated 45 minutes on ice with 80 ul of C2410-55D at 10ug/ml. 3. Cells were washed twice and incubated with S7973-88P, after which they were washed twice, fixed and analyzed by FACS. 4. A net 24% sub population of the cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a Mouse IgG1/Biotin isotype control. Binding was blocked when cells were preincubated with 20ul of 0.5mg/ml C2410-55C.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant human CD56 (truncated domains 1-4 only)

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.

Purified by Protein A affinity chromatography from tissue culture supernatant

Recognizes an epitope within the 1st four Ig-like domains of the human CD56 molecule.