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C2418-02 Mouse Anti-CD63

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P08962
Isotype
IgG1
Clone Number
MEM-259
Grade
Affinity Purified
Applications
FC IF IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets; surface expression indicates lysosomal secretion. CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release; this phenomenon has been also reported for basophils. CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmic reactions are reported. CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:25-1:100. Use 10ul to label 10e6 cells in 100ul or 100ul whole blood. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HPB-ALL cell line.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human CD63 cell surface antigen.
Conjugates
Pricing
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