Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization.

Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.

Flow Cytometry: 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry (Paraffin): Heat induced epitope retrieval is recommended prior to staining paraffin section. Sodium citrate buffer, pH 6.0 is recommended. Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions recommended. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Purified Concanavalin A acceptor glycoprotein from P815 cell line

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes mouse CD68. Detects surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.