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C2433-10E Rat Anti-CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)

Specifications
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P35762
Isotype
IgG2a
Clone Number
8H189
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Accession #
NP_598416
Gene ID
12520
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD81, also known as TAPA-1 and Tspan28, is a widely expressed protein in the Tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including Tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Mouse CD81 shares 92%-94% aa sequence identity with human and rat CD81.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 2.5ug labels 10e6 cells. Tested in mouse splenocytes Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Met1-Tyr236 from mouse CD81, expressed in NS0 cells.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse CD81. Stains mouse CD81 transfectants but not irrelevant transfectants.
Conjugates
Pricing
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