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C2438-05 Mouse Anti-CD86 (Activation B7-2 Antigen, B70, B7-2, BU63, CD28LG2, CTLA-4 Counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte Activation Antigen CD86 Precursor)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3H1726 (BU63)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in co-stimulation of T cells in the primary immune response.

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: 1:20-1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:20-1:100 Optimal dilutions to be determined by researcher.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3.X63 Ag8.653 myeloma cell line.
Positive Control Tissue (IHC)
Human Tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ARH 77 (B lymphoblastoid cell line)
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes CD86, a 70kD cell surface protein expressed by activated B cells and monocytes.
References
1. McLellan, A. et al., (1999) Immunology 98:171-180. 2. Nozawa, Y. et al. (1993). J. Pathol. 169:309.
USBio References
No references available
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