CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in co-stimulation of T cells in the primary immune response.

Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Flow Cytometry: 1:20-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry (frozen): 1:20-1:100 Optimal dilutions to be determined by researcher.

Spleen cells from immunized mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.

Human Tonsil

I1904-78R: IgG1 Murine Negative Control

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

ARH 77 (B lymphoblastoid cell line)

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes CD86, a 70kD cell surface protein expressed by activated B cells and monocytes.