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C2445-30-ML490 Mouse Anti-CD101 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
4j29 (BB27)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.
Applications: Removal of sodium azide is recommended prior to use in functional assay. Immunohistochemistry: Frozen Immunoprecipitation Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Recommended Secondary Reagents
F(ab')2 Rabbit anti mouse IgG: FITC F(ab')2 Rabbit anti mouse IgG: RPE
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG: HRP conjugate
Recommended Negative Controls
Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human thymic clone B12
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Specificity
Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).
References
1. Bagot, M. et al . (1997). CD101 is expressed by skin dendritic cells. Tissue Antigens. 50: 439 - 448.
USBio References
No references available
Conjugates
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