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C2447-35-FITC Mouse Anti-CD112 (Poliovirus Receptor Related 2, PRR2) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q92692
Conjugate
FITC
Isotype
IgG1
Clone Number
3H1817 (R2.525)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

CD112 Poliovirus Receptor Related 2 (PRR2), is a new surface molecule, which has been reported to be homologous to the poliovirus receptor (CD155). Studies demonstrate that PRR2 expression is restrictred to the myelomonocytic and megakaryocytic lineages. PRR2 expresion has also been detected on haematopoeitic progenitors expressing CD34.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Not recommended for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Recommended Secondary Antibody
I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Specificity
Recognizes human CD112.
References
1. Lopez, M. et al. (1998). The Human Poliovirus Receptor Related 2 protein is a new Hematopoietic/Endothelial Homophilic Adhesion Molecule. Blood 92: 4602-4611. 2. Goyert, S.M. (1998) White cell differentiation antigens, Myeloid Antigens: section report. Leucocyte typing VI. Kishimoto, T. et al. eds (Garland Publishing Inc, New York) p. 956-957.
USBio References
No references available
Conjugates
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