CD147 (50-60kD) has a wide tissue distribution on hematopoietic and non-hematopoietic cells including leukocytes, red blood cells, platelets and endothelial cells. As a potential adhesion molecule, CD147 may be involved in embryonic blood-brain barrier development and in integrin-mediated adhesion in brain endothelia. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemstry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-76H: Rat IgG1 Negative Control
Recommended Secondary Reagents
I1904-47Y: IgG, Fc (HRP) Pab Gt xRt I1904-47T: IgG, H&L (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt I1904-47H: IgG, H&L (HRP) Pab Gt xRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Fusion protein mouse CD147dO-2rCD4d3 +4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse CD147 (also known as basigin). Recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains.