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C2548-80H-ML650 Rat Anti-CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P18572
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
6A373 (OX114)
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CD147 (50-60kD) has a wide tissue distribution on hematopoietic and non-hematopoietic cells including leukocytes, red blood cells, platelets and endothelial cells. As a potential adhesion molecule, CD147 may be involved in embryonic blood-brain barrier development and in integrin-mediated adhesion in brain endothelia. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemstry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-76H: Rat IgG1 Negative Control
Recommended Secondary Reagents
I1904-47Y: IgG, Fc (HRP) Pab Gt xRt I1904-47T: IgG, H&L (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt I1904-47H: IgG, H&L (HRP) Pab Gt xRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
Fusion protein mouse CD147dO-2rCD4d3 +4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse CD147 (also known as basigin). Recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains.
References
1. Hanna, S.M., et al., BMC Biochemistry 4: 17 (2003). 2. Agrawal S.M. et al., Journal of Neurosci. 31:669-77 (2011). 3. Sweigard, J.H. et al., |Gene Ther. 18 6:613-21 (2011)
USBio References
No references available
Conjugates
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