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C2548-81N Mouse Anti-CD150 (SLAM, Signaling Lymphocyte Activation Molecule, IPO-3) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1,k
Clone Number
4j33 (A12 (7D4))
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.

Recommended Dilution
Suitable for use in Flow Cytometry. Other applications have not been tested.   Flow Cytometry: 5ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Activated human peripheral blood mononuclear cells.
Form
Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by affinity chromatography
Specificity
Recognizes human CD150. Species Crossreactivity: Cynomolgus, Rhesus
References
1. Cocks B, et al. 1995. Nature 376:260.|2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377.|3. Polacino P, et al. 1996. J. Med. Primatol. 25:201.|4. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
USBio References
No references available
Conjugates
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