Killer cell immunoglobulin-like receptor 2DL3 is also known as CD158b, KIR-023GB, MHC class I NK cell receptor, p58 natural killer cell receptor or Natural killer-associated transcript 2. CD158b is a 341aa, ~58 kD single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type domains that is expressed by a subset of NK cells. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. C2548-87B can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.

Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Flow Cytometry: 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.

P3UI myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

NK cell clone E57

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified

Recognizes human CD158b. It also recognizes a 50kD molecule in some NK clones which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail. Both molecules are members of the newly described natural killer cell receptor family.