CD178 cell surface antigen is a 40kD glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.

Suitable for use in Flow Cytometry (Not Tested), FLISA and Immunoprecipitation. Other applications have not been tested.

Flow Cytometry (Not Tested): 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.

Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Human CD178

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human CD178.