Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 in 100ul Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Isotype Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human erythrocytes
Form
Supplied as a liquid in Tris buffered glycine, pH 7.6, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human CD240DCE and erythrocytes of normal Rh types but does not recognize Rhnull erythrocytes. Reacts with 31kD and 35-52kD proteins which were designated CD240DCE at the 7th leukocyte typing workshop.
References
1. Mouro-Chanteloup, I. et al. (2002) Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein. Blood. 100 (3): 1038-47. 2. Mouro-Chanteloup, I. et al. (2003) Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47. Blood. 101 (1): 338-44.
TDS
NCBI
UniProt
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