CDC42BPA

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC

Crossreactivity

Bo Ca Hu Mo Rt

Accession #

NP_055641.3, NP_003598.2

Gene ID

8476, 226751, 114116

Shipping Temp

Blue Ice

Storage Temp

-20°C

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.

Applications

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:16,000 Immunohistochemistry (Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to SGEVRLRQLEQFI-C, from human CDC42BPA, at N-terminal (NP_055641.3, NP_003598.2).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human CDC42BPA. Species sequence homology: Bovine, canine, mouse and rat.

References

1. Zhao Y, Loyer P, Li H, Valentine V, Kidd V, Kraft AS. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J Biol Chem. 1997 Apr 11;272(15):10013-20.